Culture medium for avian embryonic cells
OwnerJacques Samarut, Bertrand Paininventors
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Abstract
A culture medium for avian embryonic cells and an avian cell culture medium is disclosed. The culture medium is characterized in that it has elements complementary to avian embryonic cells. The complementary elements are cytokines, fibroblast growth factors, insulin-like growth factors or stem cell growth factors. The medium is substantially free of active retinoic acid. A method for culturing avian embryonic cells, and the resulting products, are also disclosed.
Claims19
A culture medium for avian embryonic cells, said culture medium comprising basic fibroblast growth factor (bFGF), a stem cell factor (SCF), an anti-retinoic acid antibody (ARMA) that neutralizes the differentiation activity of retinoic acid in the culture medium and at least one cytokine selected from the group consisting of leukemia inhibitory factor (LIF), IL-6, IL-11, ciliary neurotrophic factor (CNTF), and Oncostatin M (OSM), wherein said culture medium is sufficient for culturing avian embryonic cells.
A method of culturing avian embryonic cells comprising:
- a) suspending avian embryonic cells originating from the blastoderm disk of an avian embryo in a culture medium comprising basic fibroblast growth factor (bFGF), a stem cell factor (SCF), an anti-retinoic acid antibody (ARMA) that neutralizes the differentiation activity of retionic acid in the culture medium and at least one cytokine selected from the group consisting of leukemia inhibitory factor (LIF), IL-6, IL-11, ciliary neutrophic factor (CNTF), and Oncostatin M (OSM);
- b) inoculating a structural support with the avian embryonic cells suspended in a), wherein said structural support is selected from the group consisting of feeder cells and gelatin;
- and
- c) culturing said avian embryonic cells.
Dependencies are inferred from the claim text.