Recombinant expression vector of human parathyroid hormone

A recombinant expression vector p153PTH which contains:

• a DNA fragment coding for 153 amino acids from amino-terminus of phosphoribulokinase of Rhodobacter sphaeroides;
• a DNA fragment coding for a urokinase-specific cleavage site of -Thr-Gly-Arg;
• and, a human parathyroid hormone gene.

The recombinant expression vector which contained the following genes in a serial manner:

• a DNA fragment coding for 153 amino acids from the amino-terminus of phosphoribulokinase of Rhodobacter sphaeroides, in which the arginine residues located at the 30th, 31th, 58th, 59th, 94th and 96th positions are substituted with different amino acids;
• a DNA fragment coding for a urokinase-specific cleavage site of X-Gly-Arg (wherein, X is Pro, Thr, Ile, Phe or Leu);
• and, a human parathyroid horomone gene.

A recombinant expression vector pm153PTH which contains the following genes in a serial manner:

• a DNA fragment coding for 153 amino acids from amino-terminus of phosphoribulokinase of Rhodobacter sphaeroides whose 30th, 58th, and 94th arginine residues are site-specifically mutated to be substituted with valines and 59th and 96th arginine residues with glycines;
• a DNA fragment coding for a urokinase-specific cleavage site of -Thr-Gly-Arg;
• and, a human parathyroid hormone gene.

A process for preparing a human parathyroid hormone which comprises the steps of:

• cultivating Escherichia coli MC1061:p153PTH (KCCM-10101);
• inducing the expression of a human parathyroid hormone by L-arabinose;
• and, recovering the human parathyroid hormone.

A process for preparing a human parathyroid hormone which comprises the steps of:

• cultivating Escherichia coli MC1061:pm153PTH (KCCM-10102);
• inducing the expression of a human parathyroid hormone by L-arabinose;
• and, recovering the human parathyroid hormone.