Inviable virus particles as scaffolds for constructing flexible detection systems
OwnerThomas L. Mattsoninventor
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Abstract
An innovative detection system for detecting small numbers of target analytes is disclosed. This system provides a novel method for attaching multiple copies of reporter groups to a single site on an analyte of interest. This system preferably comprises a virus capsid enclosing multiple detectable reporter groups, and a linking molecule which is capable of linking the capsid to the analyte of interest.
Claims34
A system for detecting an analyte comprising a non-infectious bacteriophage particle from the T Even family, a reporter group linked to said bacteriophage particle, and a linking molecule which links said bacteriophage particle to said analyte, wherein said reporter group and said linking molecule are not components of a naturally occurring virus.
A system for detecting an analyte comprising a non-infectious bacteriophage particle from the T Even family, a reporter group linked to said bacteriophage particle, said resporter group comprising a first outer capsid protein covalently linked to a first extra amino acid sequence, and a linking molecule which links said bacteriophage particle to said analyte, said linking molecule comprising a second outer capsid protein covalently linked to a second extra amino acid sequence;
- wherein said reporter group and said linking molecule are not components of a naturally occurring virus and wherein said first and second extra amino acid sequence are not identical.
A method of making a system for detecting an analyte, comprising assembling
- a. a non-infectious bacteriophage particle from the T Even family;
- b. a reporter group, said reporter group comprising a first outer capsid protein;
- c. a linking molecule which links said bacteriophage particle to said analyte, said linking molecule comprising a second outer capsid protein;
- wherein said reporter group and said linking molecule are not components of a naturally occurring virus.
Dependencies are inferred from the claim text.