Method for sequencing reaction cleanup by constant pressure diffential ultrafiltration
AssigneeMillipore Corporation
InventorsJack T. Leonard, Constance MacDonald, Joseph Gabriels
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Abstract
A method for sequencing reaction cleanup adapted to remove contaminants from a sequencing reaction, comprising the steps of: providing a defined quantity of sequencing reaction product; providing at least one ultrafiltration membrane having at least one surface; transferring the suspended sequencing reaction product to the surface of the ultrafiltration membrane; and applying a first constant pressure differential to the ultrafiltration membrane at a force capable of producing the sequencing reaction product substantially free of the contaminants.
Claims32
A method for sequencing reaction cleanup adapted to remove contaminants from a sequencing reaction, comprising the steps of,
- providing a quantity of sequencing reaction product;
- providing at least one ultrafiltration membrane having at least one surface;
- transferring said suspended sequencing reaction product to said surface of said ultrafiltration membrane;
- and
- applying a first constant pressure differential to said ultrafiltration membrane at a force capable of producing said sequencing reaction product substantially free of said contaminants, said force being between about 5 to 28 inches of mercury.
A method for sequencing reaction cleanup adapted to remove contaminants from a sequencing reaction, comprising the steps of,
- providing a quantity of sequencing reaction product;
- suspending said quantity of sequencing reaction product in one or more solvents selected from a group consisting of formamide, EDTA and water;
- providing at least one ultrafiltration membrane having at least one surface;
- transferring said suspended sequencing reaction product to said surface of said ultrafiltration membrane;
- and
- applying a first constant pressure differential to said ultrafiltration membrane at a force between about 5 to 28 inches of mercury to produce said sequencing reaction product substantially free of said contaminants.
Dependencies are inferred from the claim text.