Complementing cell lines

A packaging cell line capable of complementing recombinant adenoviruses bases on serotypes from Subgroup B, wherein said cell line is derived from primary, diploid human cells, said primary, diploid human cells being transformed by adenovirus serotype 35 E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, said sdequerces being operatively linked to regulatory seguences enabling transcription and translation of encoded proteins.

A packaging cell line capable of complementing recombinant adenoviruses bases on serotypes from Subgroup B, wherein said cell line is derived from primary, diploid human cells, said primary, diploid human cells being transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, said sequences being operatively linked to regulatory seguences enabling transcription and translation of encoded proteins, wherein the primary, diploid human cells are transformed with chimeric E1 constructs comprising E1A and E1B sequences, wherein said chimeric E1 constructs consist of E1 sequences of a serotype that enables efficient transformation of primary human cells and E1B-55K sequences of any serotype of subgroup B and E1B-21K sequences from any serotype, which E1-deleted viruses of theat serotype, and wherein said E1B sequences comprise E1B-21K and E1B-55K sequences to propagate chimeric subgroup B adenoviruses.