Use of constitutive transport elements for host range control
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsAndrea L. Ferris, Stephen H. Hughes
Life of this patent
Estimating the term…
Abstract
An Avian-derived retroviral vector from which the Constitutive Transport Element (CTE) has been removed and replaced with a genetic fragment from a mammalian virus in order to produce a vector that is replication competent in at least one non-native cell type without the need for a “helper” virus, and that is unlikely to recombine with endogenous mammalian viruses, making it both safe and simple to use in research and gene-therapy applications.
Claims13
A vector comprising an avian retrovirus, wherein a native CTE of the retovirus has been inactivated wherein all or a part of the CTE has been deleted, and into which has been inserted a nucleotide sequence comprising SEQ ID NO:
- 1, wherein the vector is replication competent in at least one cell type other than an avian retroviral host cell.
A method of making a vector that is replication competent in at least one non-native cell type comprising inactivating a CTE, wherein all or a part of a CTE sequence has been deleted from an avian-derived retrovirus and replacing the excised sequences with a a nucleotide sequence comprising the nucleotide sequence as set forth as SEQ ID NO:1, whereby the vector is replication competent in at least one cell type other than the avian retroviral host cell.
Dependencies are inferred from the claim text.