Back to results
US 6,489,155 B1Granted
Enzymes capable of degrading a sulfated-fucose-containing polysaccharide and their encoding genes
AssigneeTakara Shuzo Co., Ltd.
InventorsMasanori Takayama, Nobuto Koyama, Takeshi Sakai, Ikunoshin Kato
Life of this patent
Estimating the term…
Abstract
An isolated gene having a DNA sequence coding for the polypeptide having a degrading activity of the sulfated-fucose-containing polysaccharide or having the functionally identical activity as above.
Claims9
An isolated gene having a DNA sequence encoding a polypeptide capable of degrading a sulfated-fucose-containing polysaccharide or having a functionally identical activity, wherein said gene is selected from the following:
- (1) a gene having a DNA sequence encoding a polypeptide containing an amino acid sequence represented by either SEQ ID NO:
- 1 or SEQ ID NO:
- 2;
- wherein said polypeptide has a degrading activity which degrades a sulfated-fucose-containing polysaccharide having the following physiochemical properties:
- (a) constituting saccharide substantially free from uronic acid;
- and
- (b) substantially incapable of being degraded by the fucoidanase produced by Flavobacterium sp. SA-0082 (FERM BP-5402);
- (2) a gene having a DNA sequence encoding a polypeptide containing an amino acid sequence represented by either SEQ ID NO:
- 3 or SEQ ID NO:
- 4;
- wherein said polypeptide had a degrading activity which degrades a sulfated-fucose-containing polysaccharide having the following physiochemical properties:
- (a) constituting saccharide containing uronic acid;
- and
- (b) being degraded by the fucoidanase produced by Flavobacterium sp SA-0082 (FERM BP-5402) and degrades the said sulfated-fucose-containing polysaccharide whereby at least one of the following formulae
- is liberated;
- and
- (3) a gene having a DNA sequence which hybridizes to (1) or (2) in 6×SSC, 1% SDS, 5×Denhardt's solution and 100 μg/ml of salmon sperm DNA at 65° C.
14 more elements
An isolated polypeptide having an amino acid sequence represented by any one of SEQ ID NO:
- 1 to SEQ ID NO:
- 4;
- or having an amino acid sequence encoded by a DNA sequence which hybridizes to any one of the SEQ ID NO:
- 5 to SEQ ID NO:
- 8 in 6×SSC, 1% SDS, 5×Denhardt's solution and 100 μg/ml of salmon sperm DNA at 65° C., wherein said polypeptide has a degrading activity of either the following:
- 1. having a degrading activity which degrades a sulfated-fucose-containing polysaccharide having the following physiochemical properties:
- (a) constituting saccharide substantially free from uronic acid;
- and
- (b) substantially incapable of being degraded by the fucoidanase produced by Flavobacterium sp. SA-0082 (FERM BP-5402);
- or
- 2. having a degrading activity which acts on a sulfated-fucose-containing polysaccharide having the following physiochemical properties:
- (a) constituting saccharide containing uronic acid;
- and
- (b) being degraded by the fucoidanse produced by Flavobacterium sp SA-0082 (FERM BP-5402) and degrades the sulfated-fucose-containing polysaccharide whereby at least one of the following formulae
- is liberated.
12 more elements
Dependencies are inferred from the claim text.