Microorganisms and methods for overproduction of DAHP by cloned PPS gene
AssigneeThe Texas A&M University System
InventorJames C. Liao
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Abstract
Genetic elements comprising expression vectors and a gene coding for phosphoenol pyruvate synthase is utilized to enhance diversion of carbon resources into the common aromatic pathway and pathways branching therefrom. The overexpression of phosphoenol pyruvate synthase increases DAHP production to near theoretical yields.
Claims10
A method for increasing 3′ deoxy-D-arabino-heptulosonate (DAHB) production relative to wild type host cells, comprising the steps of:
- (a) transforming a genetic agent into a microorganism to overexpress Pps to convert pyruvate to phosphoenolpyruvate (PEP);
- and
- (b) cultivating the transformed organism in a medium.
A method for producing cyclic metabolites comprising the steps of:
- (a) transforming a genetic agent into a microorganism to overexpress Pps wherein the microorganism is a genetically engineered strain adapted to produce a desired product;
- (b) cultivating the transformed microorganism in a nutrient containing medium.
A process for the production of DAHP which comprises cultivating a microorganism in a nutrient medium and overexpressing the Pps gene.
Dependencies are inferred from the claim text.