Covalently linked oligonucleotide minor groove binder conjugates
AssigneeEpoch Biosciences, Inc.
InventorsIgor V. Kutyavin, Eugeny A. Lukhtanov, Howard B. Gamper, Rich B. Meyer, Jr.
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Abstract
Minor groove binding molecules are covalently bound to oligonucleotides which in their base sequence are complementary to a target sequence of single stranded or double stranded DNA, RNA or hybrids thereof. The covalently bound oligonucleotide minor groove binder conjugates strogly bind to the target sequence of the complementary strand.
Claims13
An oligonucleotide-minor groove binder-reporter group combination comprising an oligonucleotide having a plurality of nucleotide units, a 3′-end and a 5′-end, a minor groove binder moiety attached to at least one of said nucleotide units through a linking group which covalently binds the minor groove binder moiety to the oligonucleotide, and a reporter group;
- wherein the minor groove binder moiety is a radical of a molecule having a molecular weight of approximately 150 to approximately 2000 Daltons that binds in a non-intercalating manner into the minor groove of double stranded DNA, RNA or hybrids thereof with an association constant greater than approximately 103 M−1.
Dependencies are inferred from the claim text.