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US 6,486,304 B1Granted
Antibodies and methods of making antibodies to human thyroid protein zsig45
AssigneeZymoGenetics, Inc.
InventorsTheresa A. Deisher, Paul O. Sheppard
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Abstract
The present invention relates to polynucleotide and polypeptide molecules for zsig45, a novel human protein expressed in thyroid. The polypeptides, and polynucleotides encoding them, may be used for detecting human disease states and chromosomal abnormalities, and as a therapeutic. The present invention also includes antibodies to the zsig45 polypeptides.
Claims6
A method of producing an antibody to a polypeptide comprising:
- inoculating an animal with a polypeptide selected from the group consisting of:
- (a) a polypeptide consisting of 9 to 67 amino acids, wherein the polypeptide is identical to a contiguous sequence of amino acids in SEQ ID NO:2 from amino acid number 47 (Lys) to amino acid number 114 (Asp);
- (b) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 47 (Lys) to amino acid number 114 (Asp);
- (c) a polypeptide consisting of the amino acid sequence of SEQ ID NO:4 from amino acid number 1 (Met) to amino acid number 85 (Asp);
- (d) a polypeptide consisting of the amino acid sequence of SEQ ID NO:3 from amino acid number 1 (Met) to amino acid number 89 (Asp);
- (e) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 50 to 56;
- (f) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 61 to 66;
- (g) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 71 to 76;
- (h) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 87 to 92;
- (i) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2 from amino acid number 95 to 100;
- and
- (j) a polypeptide consisting of a hydrophilic peptide predicted from a hydrophobicity plot of the polypeptide as shown in SEQ ID NO:2 using a Hopp/Woods hydrophilicity profile based on a sliding six-residue window, with buried G, S, and T residues and exposed H, Y, and W residues ignored;
- and
- wherein the polypeptide elicits an immune response in the animal to produce the antibody;
- and
- isolating the antibody from the animal.
13 more elements
An antibody which binds to a polypeptide comprising a sequence of amino acid residues comprising an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 47 (Lys) to amino acid number 114 (Asp) of SEQ ID NO:2;
- (b) the amino acid sequence as shown in SEQ ID NO:4 from amino acid number 1 (Met) to amino acid number 85 (Asp);
- (c) the amino acid sequence as shown in SEQ ID NO:3 from amino acid number 1 (Met) to amino acid number 89 (Asp);
- (d) the amino acid sequence as shown in SEQ ID NO:2 from amino acid residue number 1 (Met) to amino acid residue number 114 (Asp).
1 more element
Dependencies are inferred from the claim text.