Human nm23-like protein
AssigneeIncyte Genomics, Inc.
InventorsOlga Bandman, Phillip R. Hawkins
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Abstract
The present invention provides polynucleotides which identify and encode a novel human nm23-like protein (H-nm23). The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding H-nm23 and for a method for producing the protein. The invention also provides for the use of substantially purified H-nm23 for the for the treatment of diseases associated with the expression of H-nm23. The invention also describes diagnostic assays which utilize diagnostic compositions comprising the polynucleotides which hybridize with naturally occurring sequences encoding H-nm23 and antibodies which specifically bind to the protein.
Claims8
An isolated polypeptide selected from the group consisting of:
- a) a polypeptide comprising the amino acid sequence of SEQ ID NO:1,
- b) a polypeptide that is at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein said polypeptide has nucleoside diphosphate kinase (NDPK) activity, and
- c) an immunogenic fragment of the polypeptide consisting of the amino acid sequence of SEQ ID NO:1, wherein said fragment is at least 15 amino acid residues in length.
A method for screening a compound for effectiveness as an agonist of the nucleoside diphosphate kinase (NDPK) activity of a polypeptide selected from the group consisting of a polypeptide comprising the amino acid sequence of SEQ ID NO:1 and a polypeptide that is at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein said polypeptide has nucleoside diphosphate kinase (NDPK) activity, the method comprising:
- a) exposing a sample comprising said polypeptide to a compound, and
- b) detecting agonist activity in the sample.
A method for screening a compound for effectiveness as an antagonist of the nucleoside diphosphate kinase (NDPK) activity of a polypeptide selected from the group consisting of a polypeptide comprising the amino acid sequence of SEQ ID NO:1 and a polypeptide that is at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein said polypeptide has nucleoside diphosphate kinase (NDPK) activity the method comprising:
- a) exposing a sample comprising said polypeptide to a compound, and
- b) detecting antagonist activity in the sample.
Dependencies are inferred from the claim text.