Oligonucleotides specific for the marORAB operon
AssigneesHybridon, Inc., Trustees of Tufts College
InventorsStuart B. Levy, Eric Von Hofe
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Abstract
Disclosed are synthetic oligonucleotides complementary to a transcript of the marORAB operon which inhibit expression of a gene in the operon. Also disclosed are methods of reducing bacterial resistance to antibiotics, and pharmaceutical formulations containing marORAB-specific oligonucleotides of the invention.
Claims21
A synthetic oligonucleotide complementary to a transcript of the marORAB operon which inhibits expression of the operon, wherein the oligonucleotide hybridizes to a sequence selected from the group consisting of:
- bases 1708-1727 of MarR, bases 1401-1450 of MarR, bases 1890-1950 of Mar A, and bases 2040-2075 of Mar A.
A method of inhibiting expression of the marORAB operon comprising contacting a transcript of the marORAB operon nucleic acid molecule with an oligonucloetide complementary to the transcript, wherein the oligonucleotide comprises a nucleotide sequence that hybridizes to a sequence selected from the group consisting of:
- bases 1708-1727 of MarR, bases 1401-1450 of MarR, bases 1890-1950 of MarA, and bases 2040-2075 of Mar A.
A method of reducing bacterial resistance to an antibiotic comprising contacting a bacterium with at least one oligonucleotide complementary to a transcript of the marORAB operon, wherein said oligonucleotide hybridizes to a sequence selected from the group consisting of:
- bases 1708-1727 of MarR, bases 1401-1450 of MarR, bases 1890-1950 of Mar A, and bases 2040-2075 of Mar A, such that bacterial resistance to an antibiotic is reduced.
A composition comprising a synthetic oligonucleotide complementary to a transcript of the marORAB operon which inhibits the expression of a locus in the operon, wherein the oligonucleotide comprises a nucleotide sequence that hybridizes to a sequence selected from the group consisting of:
- bases 1708-1727 of MarR, bases 1401-1450 of MarR, bases 1890-1950 of Mar A, and bases 2040-2075 of Mar A.
Dependencies are inferred from the claim text.