Biomaterial derived from follicle basement membranes
AssigneePurdue Research Foundation
InventorsElikplimi K. Asem, John J. Turek, J. Paul Robinson
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Abstract
A composition comprising follicle basement membrane is described. The composition can be utilized as a cell culture substrate for proliferating cells in vitro.
Claims11
A composition comprising intact basement membrane of avian ovarian follicle tissue of a vertebrate, wherein the basement membrane is substantially free of cells of said vertebrate and the basement membrane is retained in its natural three dimensional shape.
A composition comprising basement membrane of avian ovarian follicle tissue of a vertebrate, wherein the basement membrane is substantially free of cells of said vertebrate, and wherein the basement membrane is in a fluidized form.
A composition for use in supporting the growth of eukaryotic cells comprising basement membrane of avian ovarian follicle tissue of a vertebrate, wherein the basement membrane is substantially free of cells of said vertebrate, and wherein the basement membrane is in a fluidized form said composition further comprising culture-ware covered with said fluidized basement membrane.
A method for supporting the growth and differentiation of eukaryotic cells in vitro, said method comprising the step of contacting the cells in vitro with a cell growth substrate comprising basement membrane of avian ovarian follicle tissue, under conditions conducive to the growth and proliferation of said cells, wherein the basement membrane comprises a basal lamina and wherein the basal lamina is delaminated from the granulosa cells of the avian ovarian follicle tissue.
Dependencies are inferred from the claim text.