Replicating or semi-replicating viral constructs, preparation and uses for gene delivery
OwnerDavid Klatzmann, Arnaud Morel +2inventors
InventorsDavid Klatzmann, Arnaud Morel, Georg Holzer, Jean-Loup Salzmann
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Abstract
The invention relates to the use of replicating or semi-replicating viral construct(s) for the preparation of a composition for gene delivery into cells in vivo, ex vivo or in vitro. The invention also relates to novel retroviral constructs, packaging cells and nucleic acids which can be used in methods of delivering polynucleotides to cells.
Claims38
A recombinant, replicating retroviral genome, wherein said genome comprises a LTR sequence, a packaging sequence, gag, pol and env coding regions and a foreign polynucleotide, said foreign polynucleotide being inserted in said genome outside of the LTR sequence.
A recombinant replicating retroviral genome, wherein said genome comprises a LTR sequence, a packaging sequence, gag, pol and env coding regions and a foreign polynucleotide, said env coding region encoding a modified envelope glycoprotein.
A recombinant replicating viral construct, wherein said viral construct comprises a LTR sequence, a packaging sequence, gag, pol and env coding regions and a foreign polynucleotide, wherein said LTR sequence is a modified LTR sequence which is active in the presence of an activating polypeptide, and wherein said viral construct comprises a polynucleotide encoding said activating polypeptide.
A recombinant replicating viral construct, wherein said construct comprises a LTR sequence, a packaging sequence, gag, pol and env coding regions and a foreign polynucleotide, said polynucleotide encoding αGAL4.
A retrovirus packaging cell, comprising, integrated into its genome, a recombinant retroviral genome comprising at least one functional retroviral gene selected from gag, pol and env.
A composition comprising at least two retroviral constructs, for simultaneous, separated or sequential use, wherein said at least two retroviral constructs transcomplement each other when present in a cell, at least one of said retroviral constructs comprising a polynucleotide of interest.
Compositions comprising at least two semi-replicating retroviruses, for simultaneous, separated or sequential use, said semi-replicating retroviruses (i) lacking at least one viral gene selected from gag, pol and env, (ii) transcomplementing each other when present in a cell, (iii) comprising a polynucleotide and (iv) having a different envelope glycoprotein.
Compositions comprising at least three semi-replicating retroviruses, for simultaneous, separated or sequential use, said semi-replicating retroviruses (i) lacking at least one viral gene selected from gag, pol and env, (ii) transcomplementing each other when present in a cell, (iii) comprising a polynucleotide and (iv) having a different envelope glycoprotein.
Dependencies are inferred from the claim text.