Cell preconditioning and cryopresevation medium

A cell culture suspension comprising:

• eukaryotic or aerobic prokaryotic cells suspended in an aqueous medium containing:
• adenosine,
• a calcium channel blocker;
• and
• a cell nutrient matrix comprising a sufficient amount of nutrients to sustain the metabolic needs of the cells during an incubation period of at least 10 minutes without producing detectable levels of lactate or substantially depleting the nutrients so as to maintain viability of the cells, wherein the cell nutrient matrix contains a carbon and energy source, at least one vitamin selected from the group consisting of pantothenate, choline chloride, folic acid, inositol, niacinamide, pyridoxal, riboflavin and thiamine;
• and at least one amino acid selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine.

An aqueous medium for preconditioning and cryopreservation of cells harvested from a donor comprising:

• water;
• adenosine;
• a calcium channel blocker;
• and
• a cell nutrient matrix comprising a sufficient amount of nutrients to sustain the metabolic needs of the harvested cells during an incubation period of at least 10 minutes without producing detectable levels of lactate or substantially depleting the nutrients so as to maintain viability of the harvested cells, wherein the cell nutrient matrix contains 18-24 mM glucose;
• at least one vitamin selected from the group consisting of pantothenate, choline chloride, folic acid, inositol, niacinamide, pyridoxal, riboflavin and thiamine;
• and at least one amino acid selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine.

A cell suspension comprising:

• eukaryotic or aerobic prokaryotic cells suspended in an aqueous medium containing:
• adenosine;
• a calcium channel blocker;
• and
• a cell nutrient matrix comprising a sufficient amount of nutrients to sustain the metabolic needs of the cells during an incubation period of at least 10 minutes without producing detectable levels of lactate or substantially depleting the nutrients so as to maintain viability of the cells, wherein the cell nutrient matrix contains 18-24 mM glucose, at least one vitamin selected from the group consisting of pantothenate, choline chloride, folic acid, inositol, niacinamide, pyridoxal, riboflavin and thiamine, and at least one amino acid selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glycine, histidine, isoleucine, leucine, lysine, metonine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine.

An aqueous medium for preconditioning and cryopreservation of cells harvested from a donor comprising:

• water;
• from about 2.7 mM to about 3.6 mM adenosine;
• from about 0.04 mM to about 0.07 mM verapamil;
• a cryoprotectant;
• and
• a cell nutrient matrix comprising a sufficient amount of nutrients to sustain the metabolic needs of harvested cells when the harvested cells are maintained in oxygenated aqueous medium at a temperature of from about 35° to about 40° C. for a period of from about ten minutes to about 2 hours without producing detectable levels of lactate or substantially depleting the nutrients so as to maintain the viability of the harvested cells, wherein the cell nutrient matrix contains an energy source and at least one vitamin selected from the group consisting of pantothenate, choline chloride, folic acid, inositol, niacinamide, pyridoxal, riboflavin, and thiamine.