Testis-specific human SVPH1-8 proteinase
AssigneeImmunex Corporation
InventorDouglas P. Cerretti
Life of this patent
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Abstract
The disclosure provides purified and isolated SVPH1-8 polypeptides, nucleic acids encoding such polypeptides, processes for production of recombinant forms of such polypeptides, antibodies generated against such polypeptide, and fragmented peptides derived from these polypeptide. In addition, the disclosure provides uses of such polypeptides, fragmented peptides, antibodies and nucleic acids as well as kits containing the foregoing.
Claims34
An isolated polypeptide comprising a sequence selected from the group consisting of:
- (a) SEQ ID NO:2;
- (b) a fragment of SEQ ID NO:2, such that a polypeptide with an amino acid sequence consisting of the fragment has proteinase activity;
- and
- (c) a variant amino acid sequence that is at least 90% identical to SEQ ID NO:2 or a fragment thereof, such that a polypeptide consisting of the variant amino acid sequence or said fragment has proteinase activity.
An isolated polypeptide comprising an SVPH1-8 variant, wherein the SVPH1-8 variant has proteinase activity and is encoded by a nucleic acid sequence that hybridized under high stringency in 50% formamide and 6×SSC, at 42° C. with washing conditions of 68° C., 0.2×SSC, 0.1% SDS to the complement of a nucleic acid consisting of the sequence of SEQ ID NO:1.
A polypeptide produced by a method comprising culturing a host cell containing a recombinant nucleic acid molecule encoding the polypeptide under conditions promoting expression of the polypeptide from the recombinant nucleic acid molecule, wherein the recombinant nucleic acid molecule comprises a sequence selected from the group consisting of:
- (a) SEQ ID NO:1;
- (b) a fragment of SEQ ID NO:1 such that a nucleic acid molecule with a nucleotide sequence consisting of the fragment encodes a polypeptide having proteinase activity;
- (c) a nucleic acid sequence encoding an SVPH1-8 variant having proteinase activity, wherein the nucleic acid sequence hybridizes under high stringency in 50% formamide and 6×SSC, at 42° C. with washing conditions of 68° C., 0.2×SSC, 0.1% SDS to the complement of a nucleic acid consisting of SEQ ID NO:1;
- and
- (d) a nucleotide sequence that is degenerate as a result of the genetic code to a sequence of (a), (b), or (c) and encodes a polypeptide that has proteinase activity.
A fusion polypeptide comprising a first polypeptide and a second polypeptide, wherein the first polypeptide comprises a sequence selected from the group consisting of:
- (a) SEQ ID NO:2;
- (b) a fragment of SEQ ID NO:2, such that a polypeptide with an amino acid sequence consisting of the fragment has proteinase activity;
- and
- (c) a variant amino acid sequence that is at least 90% identical to SEQ ID NO:2 or a fragment thereof, such that a polypeptide consisting of the variant amino acid sequence or said fragment has proteinase activity.
An isolated polypeptide comprising a sequence selected from the group consisting of:
- (a) a fragment of SEQ ID NO:2 such that a polypeptide with an amino acid sequence consisting of the fragment has disintegrin activity;
- and
- (b) a variant amino acid sequence that is at least 90% identical to SEQ ID NO:2 or a fragment thereof, such that a polypeptide consisting of the variant amino acid sequence or said fragment has disintegrin activity.
An isolated polypeptide comprising an SVPH1-8 variant, wherein the SVPH1-8 variant has disintegrin activity and is encoded by a nucleic acid sequence that hybridizes under high stringency in 50% formamide and 6×SSC, at 42° C. with washing conditions of 68° C., 0.2×SSC, 0.1% SDS to the complement of a nucleic acid consisting of the sequence of SEQ ID NO:1.
A polypeptide produced by a method comprising culturing a host cell containing a recombinant nucleic acid molecule encoding the polypeptide under conditions promoting expression of the polypeptide from the recombinant nucleic acid molecule, wherein the recombinant nucleic acid molecule comprises a sequence selected from the group consisting of:
- (a) a fragment of SEQ ID NO:1 such that a nucleic acid molecule with a nucleotide sequence consisting of the fragment encodes a polypeptide having disintegrin activity;
- (b) a nucleic acid sequence encoding an SVPH1-8 variant having disintegrin activity, wherein the nucleic acid sequence hybridizes under high stringency in 50% formamide and 6×SSC, at 42° C. with washing conditions of 68° C., 0.2×SSC, 0. 1% SDS to the complement of a nucleic acid consisting of SEQ ID NO:1;
- and
- (c) a nucleotide sequence that is degenerate as a result of the genetic code to a sequence of (a) or (b) and encodes a polypeptide that has disintegrin activity.
A fusion polypeptide comprising a first polypeptide and a second polypeptide, wherein the first polypeptide comprises a sequence selected from the group consisting of:
- (a) a fragment of SEQ ID NO:2 such that a polypeptide with an amino acid sequence consisting of the fragment has disintegrin activity;
- and
- (b) a variant amino acid sequence that is at least 90% identical to SEQ ID NO:2 or a fragment thereof, such that a polypeptide consisting of the variant amino acid sequence or said fragment has disintegrin activity.
Dependencies are inferred from the claim text.