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US 6,485,954 B1Granted
Enzyme with galactanase activity
AssigneeNovozymes A/S
InventorsLene Venke Kofod, Markus Sakari Kauppinen, Lene Nonboe Andersen, Ib Groth Clausen, Anette Müllertz
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Abstract
The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme with galactanase activity, a method of producing the enzyme, an enzyme composition comprising said enzyme with galactanase activity, and the use of said enzyme and enzyme composition for a number of industrial applications.
Claims36
An isolated polypeptide exhibiting galactanase activity selected from the group consisting of:
- (a) a polypeptide encoded by the galactanase enzyme encoding part of the DNA sequence cloned into plasmid pYES 2.0 present in Saccharomyces cerevisiae DSM 9983;
- (b) a polypeptide comprising an amino acid sequence of amino acid residues 19-350 of SEQ ID NO:
- 2 or a fragment thereof that exhibits galactanase activity;
- and
- (c) a polypeptide which has an amino acid sequence that is at least 80% homologous with the polypeptide of amino acid residues 19-350 of SEQ ID NO:
- 2, wherein the homology is determined using GAP with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1;
- and
- (d) a polypeptide encoded by a DNA sequence that hybridizes with SEQ ID NO:
- 1 under high stringency conditions, wherein the conditions are defined as prehybridization in a solution of 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 μg/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution containing a concentration of 10 ng/ml of a random-primed 32P-dCTP-labeled probe for 12 hours at a temperature of about 45° C. followed by washing for 30 minutes in 2×SSC, 0.5% SDS at a temperature of at least 70° C.
6 more elements
An isolated enzyme exhibiting galactanase activity selected from the group consisting of:
- (a) a polypeptide encoded by the galactanase enzyme encoding part of the DNA sequence cloned into plasmid pYES 2.0 present in Saccharomyces cerevisiae DSM 9976;
- (b) a polypeptide comprising an amino acid sequence of amino acid residues 19-349 of SEQ ID NO:
- 4 or a fragment thereof having galactanase activity;
- (c) a polypeptide having an amino acid sequence which is at least 80% homologous with the polypeptide of amino acid residues 19-349 of SEQ ID NO:
- 4, wherein the homology is determined using GAP with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1;
- and
- (d) a polypeptide encoded by a DNA sequence that hybridizes with SEQ ID NO:
- 3 under high stringency conditions, wherein the conditions are defined as prehybridization in a solution of 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 μg/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution containing a concentration of 10 ng/ml of a random-primed 32P-dCTP-labeled probe for 12 hours at a temperature of about 45° C. followed by washing for 30 minutes in 2×SSC, 0.5% SDS at a temperature of at least 70° C.
5 more elements
Dependencies are inferred from the claim text.