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US 6,485,952 B1Granted
Method of cultivating white-rot fungi on a sugar beet pulp substrate
AssigneeEmerald BioAgriculture Corporation
InventorsClifford A. Bradley, Robert D. Kearns, Pauline P. Wood, William E. Black
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Abstract
White-rot fungi are grown on a sugar beet pulp substrate. By-product of fungal growth, such as lignin-degrading enzymes, can be recovered from the culture.
Claims9
A method of culturing white-rot fungus, comprising
- combining a white-rot fungus and a sugar beet pulp substrate such that the white-rot fungus produces lignin degrading enzyme during primary metabolism and the sugar beet pulp substrate has a total nitrogen content based on dry weight of about ten percent forming a high nitrogen fungal culture;
- subjecting the high nitrogen fungal culture to growth-supportive conditions such that a lignin degrading enzyme is produced by the white-rot fungus during a growing period, wherein the growing period includes primary and secondary metabolism and a lignin degrading enzyme is produced during primary metabolism;
- and
- recovering the lignin degrading enzyme.
1 more element
A method for producing lignin degrading enzymes, comprising:
- combining a white-rot fungus and a sugar beet pulp substrate in a high nitrogen fungal culture;
- and
- subjecting the high nitrogen fungal culture to growth-supportive conditions such that a lignin degrading enzyme is produced by the white-rot fungus during primary metabolism of the fungal growth period;
- and
- recovering a lignin degrading enzyme.
2 more elements
Dependencies are inferred from the claim text.