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AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsGeorge J. Piazza, Alberto Nunez, Thomas A. Foglia
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A method has been discovered for the epoxidation of a compound having at least one carbon-carbon double bond, the method involves reacting a compound having at least one carbon-carbon double bond, a solvent, an oxidant, and membrane bound peroxygenase. Also discovered is a method for preparing the membrane bound peroxygenase involving grinding seeds containing peroxygenase to produce ground seeds, homogenizing the ground seeds in a buffer to form a slurry, centrifuging the slurry to produce a first supernatant, centrifuging the first supernatant to produce a second supernatant, and filtering said second supernatant through a protein-binding membrane filter to produce membrane bound peroxygenase; optionally the second supernatant is filtered through a hydrophilic membrane filter prior to filtering the second supernatant through a protein-binding membrane filter.
A method for preparing membrane bound peroxygenase, said method comprising grinding seeds containing peroxygenase to produce ground seeds, homogenizing said ground seeds in a buffer to form a slurry, centrifuging said slurry to produce a first supernatant, centrifuging said first supernatant to produce a second supernatant, and filtering said second supernatant through a protein-binding membrane filter to produce membrane bound peroxygenase, wherein said protein-binding membrane bas a pore size of about 0.1 μm to about 2 μm.
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