Heat shock transcription factor-binding protein
AssigneeHSP Research Institute, Inc.
InventorsTadahiko Yoshima, Hideki Yanagi, Takashi Yura
Life of this patent
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Abstract
To provide a heat shock transcription factor (HSF) 2 binding factor, which can be involved in the transcriptional regulation of HSP70.2 playing an essential role in spermatogenesis; a DNA encoding the binding factor; an expression vector carrying the DNA; a transformant harboring the expression vector; a process for preparing a recombinant protein comprising the step of culturing the transformant; an antibody or a fragment thereof capable of specifically binding to the binding factor; an antisense DNA or antisense RNA complementary to the DNA; and an oligonudeotide probe or primer capable of specifically hybridizing to the DNA.
Claims7
An isolated DNA encoding a protein having a heat shock transcription factor (HSF) 2 binding activity, which is selected from the group consisting of:
- (a) a DNA encoding a peptide comprising the amino acid sequence of SEQ ID NO:1;
- (b) a DNA comprising the nucleotide sequence of SEQ ID NO:2;
- and
- (c) a DNA capable of hybridizing with a DNA of any one of (a) to (b), under the following stringent conditions:
- heating at 42° C. in a solution containing 6×SSC, 0.5% SDS and 50% formamide, followed by washing at 68° C. in a solution containing 0.1×SSC and 0.5% SDS.
An isolated antibody or a fragment thereof which specifically binds to the protein of SEQ ID NO:1.
An antisense DNA or antisense RNA having a sequence that is complementary to the DNA of SEQ ID NO:2.
Dependencies are inferred from the claim text.