Structure of the ankyrin binding domain of a α-Na, K-ATPase
AssigneeYale University
InventorsJon S. Morrow, Prasad Devarajan, Zhushan Zhang
Life of this patent
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Abstract
The present invention provides a polypeptide of 26 amino acid residues that comprises the minimal ankyrin binding (MAB) domain. The MAB domain is responsible for the interactions between a Na,K-ATPase and ankyrin. The present invention also provides the three dimensional structure of the MAB domain. Also provided by the present invention are methods for modulating the interaction of a Na,K-ATPase and ankyrin.
Claims18
An isolated peptide of 8, 9, 10, 15, 20, 25 or 26 amino acid residues comprising the minimal ankyrin binding domain amino acid sequence of SEQ ID NO:
- 1.
An isolated peptide of 8, 9, 10, 15, 20, 25 or 26 amino acid residues comprising the minimal ankyrin binding domain amino acid sequence X1 X2 Ser Phe X5 X6 X7 (SEQ ID NO:
- 3) wherein:
- (a) X1 is a nonpolar amino acid selected from the group consisting of methionine and isoleucine;
- (b) X2 is a polar uncharged or negatively charged amino acid, selected from the group consisting of glutamic acid, aspartic acid and serine;
- (c) X5 and X6 are either polar uncharged or positively charged amino acids selected from the group consisting of lysine and asparagine;
- (d) X7 is a nonpolar amino acid selected from the group consisting of methionine and leucine.
An isolated peptide comprising the amino acid sequence of SEQ ID NO:
- 1 which blocks the activity of alpha-Na,K ATPase by modulating the binding of alpha-Na,K ATPase to ankyrin.
An isolated peptide which blocks the activity of alpha-Na,K ATPase by modulating the binding of the alpha-Na,K ATPase to ankyrin comprising the sequence X1 X2 Ser Phe X5 X6 X7 (SEQ ID NO:
- 3) wherein:
- (a) X2 is a nonpolar amino acid selected from the group consisting of methionine and isoleucine;
- (b) X2 is a polar uncharged or negatively charged amino acid, selected from the group consisting of glutamic acid, aspartic acid and serine;
- (c) X5 and X6 are either polar uncharged or positively charged amino acids selected from the group consisting of lysine and asparagine;
- (d) X7 is a nonpolar amino acid selected from the group consisting of methionine and leucine.
An isolated peptide consisting of SEQ ID NO:
- 1.
An isolated peptide consisting of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.