Loading patent…
OwnerNico Maurice August Corneel Mertens, Eric Rene Remaut +1inventors
InventorsNico Maurice August Corneel Mertens, Eric Rene Remaut, Walter Charles Fiers
Estimating the term…
The invention relates to a regulation system for inducible expression of genes, comprising a lambdoid promoter, a gene coding for a repressor for the lambdoid promoter and a gene coding for an antirepressor of the repressor, which antirepressor is under the influence of an inducible promoter. The invention further relates to a regulatory replicon, comprising said gene coding for an antirepressor, an expression system, comprising said regulatory replicon, and an expression vector based on a lambdoid promoter, and also to a method for producing a gene product in a heterologous host, by providing a culture of a host comprising a heterologous sequence which codes for the gene product. Providing a culture of a host comprising a heterologous sequence is obtained by putting the expression of the heterologous sequence under the control of a regulation system, a gene coding for a repressor for the lambdoid promoter and a gene coding for an antirepressor, and by inducing the promoter of the antirepressor gene.
The polynucleotide components for inducible expression of a gene or genes of interest (GOI) comprising:
An isolated regulatory replicon, comprising a gene coding for the ant protein of lambdoid phage p22, wherein said gene coding for the ant protein of lambdoid phage p22 is under the control of an inducible promoter, and wherein said ant protein of lambdoid phage p22 represses the action of a repressor of a lambdoid promoter by binding to the repressor;
A method for producing a gene product in a heterologous host, comprising:
Dependencies are inferred from the claim text.