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US 6,485,924 B2Granted
Method for identifying inhibitors of cytokinesis
AssigneeBoehringer Ingelheim International GmbH
InventorsManuel Mendoza, Michael Glotzer
Life of this patent
Estimating the term…
Abstract
Method for identifying compounds that inhibit cytokinesis, wherein the compounds are tested for their ability to modulate the formation and/or the stability of septin filaments. The compounds are useful in tumor therapy.
Claims48
A method for identifying compounds that modulate cytokinesis comprising:
- (a) in a control system, incubating septin molecules under conditions that allow polymerization of the septin molecules, wherein said conditions include the presence of GTP;
- (b) in a test system, incubating septin molecules under the conditions of (a), wherein said incubation occurs in the presence of test compounds;
- (c) measuring GTP hydrolysis and/or binding in (a) and (b);
- and
- (d) comparing the GTP hydrolysis and/or binding in (a) with that of (b), wherein an enhancement of GTP hydrolysis and/or binding or a decrease in GTP hydrolysis and/or binding demonstrates that said test compound has the ability to modify cytokinesis.
2 more elements
A method for identifying compounds that modulate cytokinesis comprising:
- (a) in a system, incubating septin molecules under conditions that allow polymerization of the septin molecules, wherein said conditions include the presence of GTP;
- (b) measuring GTP hydrolysis and/or binding in (a);
- (c) adding test compounds to said system (a) after polymerization;
- (d) measuring GTP hydrolysis and/or binding in said system after step (c);
- and
- (e) comparing the GTP hydrolysis and/or binding in (b) with that of (d), wherein an enhancement of GTP hydrolysis and/or binding or a decrease in GTP hydrolysis and/or binding demonstrates that said test compound has the ability to modify cytokinesis.
3 more elements
A method for identifying compounds that inhibit cytokinesis comprising:
- (a) in a control system, incubating septin molecules under conditions that allow polymerization of the septin molecules, wherein said conditions include the presence of GTP;
- (b) in a test system, incubating septin molecules under the conditions of (a), wherein said incubation occurs in the presence of test compounds;
- (c) measuring GTP hydrolysis and/or binding in (a) and (b);
- and
- (d) comparing the GTP hydrolysis and/or binding in (a) with that of (b), wherein a decrease of GTP hydrolysis and/or binding demonstrates that said test compound has the ability to inhibit cytokinesis.
2 more elements
A method for identifying compounds that enhance cytokinesis comprising:
- (a) in a control system, incubating septin molecules under conditions that allow polymerization of the septin molecules, wherein said conditions include the presence of GTP;
- (b) in a test system, incubating septin molecules under the conditions of (a), wherein said incubation occurs in the presence of test compounds;
- (c) measuring GTP hydrolysis and/or binding in (a) and (b);
- and
- (d) comparing the GTP hydrolysis and/or binding in (a) with that of (b), wherein an enhancement of GTP hydrolysis and/or binding demonstrates that said test compound has the ability to enhance cytokinesis.
2 more elements
A method for identifying compounds that inhibit cytokinesis comprising:
- (a) in a system, incubating septin molecules under conditions that allow polymerization of the septin molecules, wherein said conditions include the presence of GTP;
- (b) measuring GTP hydrolysis and/or binding in (a);
- (c) adding test compounds to said system (a) after polymerization;
- (d) measuring GTP hydrolysis and/or binding in said system after step (c);
- and
- (e) comparing the GTP hydrolysis and/or binding in (b) with that of(d), wherein a decrease in GTP hydrolysis and/or binding demonstrates that said test compound has the ability to inhibit cytokinesis.
3 more elements
A method for identifying compounds that enhance cytokinesis comprising:
- (a) in a system, incubating septin molecules under conditions that allow polymerization of the septin molecules, wherein said conditions include the presence of GTP;
- (b) measuring GTP hydrolysis and/or binding in (a);
- (c) adding test compounds to said system (a) after polymerization;
- (d) measuring GTP hydrolysis and/or binding in said system after step (c);
- and
- (e) comparing the GTP hydrolysis and/or binding in (b) with that of (d), wherein an enhancement of GTP hydrolysis and/or binding demonstrates that said test compound has the ability to enhance cytokinesis.
3 more elements
Dependencies are inferred from the claim text.