Preparation method of nucleic acid sample for rare expressed genes and analyzing method using the prepared nucleic acid samples thereby
AssigneeHitachi, Ltd.
InventorsTakamichi Muramatsu, Takeshi Fujita, Masaharu Kiyama, Takashi Irie, Kazunori Okano
Life of this patent
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Abstract
Focusing that abundant class genes abundantly present in a nucleic acid sample can comparatively easily be analyzed and readily be removed in a selective manner, a nucleic acid sample for expression analysis of rare expressed genes can be obtained by removing abundant genes therefrom, and analyses based upon the sample can be achieved.
Claims8
An apparatus for removing one or plural genes hybridized with probes from a mixture of one or plural abundant expressed genes hybridized with probes and rare expressed genes not hybridized with probes, comprising:
- a first chamber having a lid at a top part and a first membrane filter at a lower part, a solution containing a mixture of one or plural abundant expressed genes hybridized with the probes is added into said first chamber, said first membrane filter chemically or physically retaining said abundant expressed genes hybridized with the probes, and said first membrane filter having a pore size permeating said rare expressed genes not hybridized with the probes therethrough;
- a second chamber having a second membrane filter at a lower part and coupled to a lower part of said first chamber, said second membrane filter not permeating said rare expressed genes not hybridized with the probes and permeating water and inorganic salts therethrough to concentrate said rare expressed genes not hybridized with the probes;
- and
- a third chamber coupled to a lower part of said second chamber, substances permeated through said second membrane filter being collected into said third chamber;
- wherein said abundant expressed genes hybridized with the probes remain in said first chamber and said rare expressed genes not hybridized with the probes remain in said second chamber.
An electrophoresis cell for removing one or plural genes hybridized with probes from a mixture of one or plural abundant expressed genes hybridized with probes and rare expressed genes not hybridized with probes, comprising:
- a first cell in which a buffer solution is contained and a first electrode is provided;
- a second cell in which the buffer solution is contained;
- and
- a third cell in which the buffer solution is contained and a second electrode is provided;
- wherein said first cell and said second cell have a common first membrane filter for dividing said first cell and said second cell, said second cell and said third cell have a common second membrane filter for dividing said second cell and said third cell, a solution containing a mixture of one or plural abundant expressed genes hybridized with probes and rare expressed genes not hybridized with the probes is added into said first cell, said common first membrane filter chemically or physically retaining said abundant expressed genes hybridized with the probes, and said common first membrane filter having a pore size permeating said rare expressed genes not hybridized with the probes therethrough;
- wherein said common second membrane filter do not permeate said rare expressed genes not hybridized with the probes;
- and
- wherein, by supplying an electric potential between the first and second electrode and performing electrophoresis for migrating said rare expressed genes not hybridized with the probes from the first cell to the second cell, said abundant expressed genes hybridized with the probes remain in said first cell, and said rare expressed genes not hybridized with the probes remain in said second cell.
Dependencies are inferred from the claim text.