Method of detecting chlamydia pneumoniae gene using polynucleotides

A method for detecting Chlamydia pneumoniae gene in a biological sample, comprising the steps of:

• labeling a probe, wherein said probe comprises any one of:
• (i) a polynucleotide having at least ten consecutive bases of a Chlamydia pneumoniae-specific sequence selected from the group consisting of SEQ ID NO 3, SEQ ID NO 19, and SEQ ID NO 20;
• (ii) a polynucleotide complementary to said polynucleotide in (i);
• or
• (iii) a polynucleotide having at least 90% homology to said polynucleotide in (i) or (ii),
• isolating nucleic acids in a biological sample,
• transferring said isolated nucleic acids to a filter, hybridizing said labeled probe with said nucleic acids on said filter, and
• detecting a complex as formed with said labeled probe and said isolated nucleic acids on said filter, wherein said complex indicates the presence of said Chlamydia pneumoniae gene in said biological sample.

A method for detecting Chlamydia pneumoniae gene in a biological sample, comprising the steps of:

• (a) providing a pair of PCR primers, wherein at least one of said PCR primers comprises any one of:
• (i) a polynucleotide having at least ten consecutive bases of Chlamydia pneumoniae-specific sequence selected from the group consisting of SEQ ID NO 3, SEQ ID NO 19, and SEQ ID NO 20;
• (ii) a polynucleotide complementary to said polynucleotide in (i);
• or
• (iii) a polynucleotide having at least 90% homology to said polynucleotide in (i) or (ii),
• (b) contacting a biological sample with said primer,
• (c) amplifying a nucleic acid product, and
• (d) detecting said nucleic acid product, wherein said nucleic acid product indicates the presence of said Chlamydia pneumoniae gene in said biological sample.

A method for detecting Chlamydia pneumoniae gene in a biological sample by PCR, comprising the steps of:

• (a) adding a DNA polymerase to a biological sample;
• (b) mixing said biological sample with a pair of primers to form a mixture, wherein at least one of said primer comprises any one of
• (i) a polynucleotide having at least ten consecutive bases of a Chlamydia pneumoniae-specific sequence selected from the group consisting of SEQ ID NO 3, SEQ ID NO 19, and SEQ ID NO 20;
• (ii) a polynucleotide complementary to said polynucleotide in (i);
• or
• (iii) a polynucleotide having at least 90% homology to said polynucleotide in (i) or (ii),
• (c) heating said mixture to a first temperature for a first period of time;
• (d) cooling said mixture to a second temperature for a second period of time;
• (e) keeping said mixture at a third temperature for a third period of time;
• (f) repeating steps (c) to (e) for a plurality of times, and
• (g) detecting a nucleic acid product of the step (f) in said mixture, wherein said nucleic acid product indicates the presence of said Chlamydia pneumoniae gene in said biological sample.