Back to results
US 6,485,907 B1Granted
PCR-based detection of Rhizoctonia cerealis
AssigneeSyngenta Participations AG
InventorsJames Joseph Beck, Charles Jason Barnett
Life of this patent
Estimating the term…
Abstract
Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for different strains of the wheat fungal pathogen, Rhizoctonia cerealis. Specific primers from within these sequences are identified as being useful for the identification of Rhizoctonia cerealis using PCR-based techniques.
Claims15
An isolated DNA molecule comprising an Internal Transcribed Spacer sequence selected from the group consisting of:
- (a) ITS1 of Rhizoctonia cerealis, wherein ITS1 of Rhizoctonia cerealis comprises nucleotides 31-243 of SEQ ID NO:17, nucleotides 31-242 of SEQ ID NO:18, nucleotides 31-243 of SEQ ID NO:19, nucleotides 31-241 of SEQ ID NO:20, nucleotides 31-243 of SEQ ID NO:21, nucleotides 31-243 of SEQ ID NO:22, nucleotides 31-180 of SEQ ID NO:23, nucleotides 31-240 of SEQ ID NO:24, nucleotides 31-242 of SEQ ID NO:25, or nucleotides 31-242 of SEQ ID NO:26;
- and
- (b) ITS2 of Rhizoctonia cerealis, wherein ITS2 of Rhizoctonia cerealis comprises nucleotides 397-630 of SEQ ID NO:21, nucleotides 397-630 of SEQ ID NO:22, nucleotides 397-630 of SEQ ID NO:23, nucleotides 394-627 of SEQ ID NO:24, or nucleotides 396-629 of SEQ ID NO:26.
An oligonucleotide primer selected from the group consisting of SEQ ID NO's:7-16.
Dependencies are inferred from the claim text.