DNA encoding a plasminogen activating protein

An isolated polynucleotide molecule that hybridizes to a DNA molecule comprising a nucleotide sequence complementary to the nucleotide sequence from nucleotide position 195 to nucleotide position 447 of SEQ ID NO:1, or from nucleotide position 196 to nucleotide position 448 of SEQ ID NO:3, under conditions of 0.5M NaHPO4, 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at 65° C., and washing in 0.2×SSC/0.1% SDS at 42° C., and which polynucleotide molecule encodes a polypeptide that, when administered to a member of a mammalian species, is capable of inducing the production of antibodies that bind specifically to a protein comprising the amino acid sequence from amino acid position 26 to amino acid position 286 of SEQ ID NO:2 or SEQ ID NO:4.

An isolated polynucleotide molecule, comprising a nucleotide sequence encoding a peptide fragment of a protein consisting of the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4, which peptide fragment, when administered to a member of a mammalian species, is capable of inducing the production of antibodies that bind specifically to a protein comprising the amino acid sequence from amino acid position 26 to amino acid position 286 of SEQ ID NO:2 or SEQ ID NO:4.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding a fusion protein, said fusion protein comprising a fusion partner fused to a protein comprising the amino acid sequence from amino acid position 26 to amino acid position 286 of SEQ ID NO:2 or SEQ ID NO:4.

An isolated polynucleotide molecule comprising a nucleotide sequence encoding a fusion protein, said fusion protein comprising a fusion partner fused to a peptide fragment of a protein consisting of the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4, which peptide fragment, when administered to a member of a mammalian species, is capable of inducing the production of antibodies that bind specifically to a protein comprising the amino acid sequence from amino acid position 26 to amino acid position 286 of SEQ ID NO:2 or SEQ ID NO:4.

A recombinant expression vector, comprising a polynucleotide molecule comprising a nucleotide sequence encoding:

• (a) a protein comprising the amino acid sequence from amino acid position 26 to amino acid position 286 of SEQ ID NO:2 or SEQ ID NO:4;
• or (b) a fusion protein comprising a fusion partner fused to the protein of (a);
• which polynucleotide molecule is in operative association with one or more regulatory elements that control expression of the polynucleotide molecule in a host cell.

A recombinant expression vector, comprising a polynucleotide molecule consisting of a nucleotide sequence encoding:

• (a) a peptide fragment of a protein consisting of the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4, which peptide fragment, when administered to a member of a mammalian species, is capable of inducing the production of antibodies that bind specifically to a protein comprising the amino acid sequence from amino acid position 26 to amino acid position 286 of SEQ ID NO:2 or SEQ ID NO:4;
• or (b) a fusion protein comprising a fusion partner fused to the peptide fragment of (a);
• which polynucleotide molecule is in operative association with one or more regulatory elements that control expression of the polynucleotide molecule in a host cell.

A method for determining whether an animal is infected with Streptococcus uberis, comprising analyzing a tissue or fluid sample from the animal for the presence of a polynucleotide molecule that encodes a protein comprising the amino acid sequence from amino acid position 26 to amino acid position 286 of SEQ ID NO:2 or SEQ ID NO:4.