Methods for inhibiting angiogenesis with leukocyte adhesion inhibitor-1 (LAI-1) polypeptides
AssigneeHuman Genome Sciences, Inc.
InventorsHaodong Li, Brent L. Kreider
Life of this patent
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Abstract
There are disclosed therapeutic compositions and methods using isolated nucleic acid molecules encoding a human chemokine beta-11 (Ck beta-11) polypeptide and a human leukocyte adhesion inhibitor-1 (LAI-1) polypeptide (previously termed chemokine α1(CKα1 or cka-1), as well as Ck beta-11 and/or LAI-1 polypeptides themselves, as are vectors, host cells and recombinant methods for producing the same.
Claims15
A method of inhibiting angiogenesis, comprising administering to an animal an effective amount of a polypeptide comprising an anti-angiogenic fragment of SEQ ID NO:4.
A method of inhibiting angiogenesis comprising administering to an animal an effective amount of a polypeptide comprising an anti-angiogenic polypeptide comprising an amino acid sequence of SEQ ID NO:4 selected from the group consisting of:
- 1. Gly (22)-Pro (109);
- 2. Val (23)-Pro (109);
- 3. Leu (24)-Pro (109);
- 4. Glu (25)-Pro (109);
- 5. Val (26)-Pro (109);
- 6. Tyr (27)-Pro (109);
- 7. Tyr (28)-Pro (109);
- 8. Thr (29)-Pro (109);
- 9. Ser (30)-Pro (109);
- 10. Gly (22)-Pro (109);
- 11. Val (23)-Ile (109);
- 12. Leu (24)-Ile (108);
- 13. GIu (25)-Lys (107);
- 14. Val (26)-Arg (106);
- 15. Tyr (27)-Lys (105);
- 16. Tyr (28)-Phe (104);
- 17. Thr (29)-Val (103);
- 18. Val (23)-Pro (102);
- 19. Val (23)-Val (101);
- 20. Val (23)-Pro (100);
- 21. Val (23)-Leu (99);
- 22. Val (23)-Thr (98);
- 23. Val (23)-Ser (97);
- 24. Val (23)-Ser (96);
- 25. Val (23)-Ser (95);
- 26. Val (23)-Arg (94);
- 27. Val (23)-Lys (93);
- 28. Val (23)-Arg (92);
- 29. Val (23)-Leu (91);
- 30. Val (23)-Val (90);
- 31. Val (23)-Glu (89);
- 32. Val (23)-Met (88);
- 33. Val (23)-Met (87);
- 34. Val (23)-Arg (86);
- 35. Val (23)-Gln (85);
- 36. Val (23)-Ile (84);
- 37. Val (23)-Trp (83);
- 38. Val (23)-Glu (82);
- 39. Val (23)-Ala (81);
- 40. Val (23)-Gln (80);
- 41. Val (23)-Pro (79);
- 42. Val (23)-Asp (78);
- 43. Val (23)-Val (77);
- 44. Val (23)-Cys (76);
- 45. Ser (30)-Lys (107);
- 46. Ser (30)-Lys (105);
- 47. Ser (30)-Lys (93);
- and
- 48. Ser (30)-Cys(76).
A method of inhibiting anglogenesis, comprising administering to an animal an effective amount of a polypeptide comprising an anti-angiogenic fragment of the amino acid sequence encoded by the cDNA clone of ATCC Deposit No. 75947.
Dependencies are inferred from the claim text.