Compositions and methods for analyte detection
AssigneesThe Texas A&M University System, The General Hospital Corporation
InventorsGerard L. Coté, Michael V. Pishko, Kaushik Sirkar, Ryan Russell, Richard Rox Anderson
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Abstract
Provided are a variety of chemically sensitive, stable (insoluble over a specified period of time), nontoxic, and non-antigenic hydrogel particles which undergo a measurable change in at least one electrochemical or optical property as a function of interaction with one or more substance(s) to be detected. Also provided are methods of using these hydrogel particles to detect one or more selected analytes, and in certain aspects detect one or more analytes in vivo. Further provided are devices used to detect and measure the optical or electrochemical changes.
Claims11
A method for the detection of an analyte in an animal, wherein the analyte is glucose, comprising the steps of:
- (a) implanting by injecting within the epidermis or dermis of the animal a composition comprising a hydrogel and an analyte detection compound, wherein said hydrogel comprises a cross-linked poly(ethylene glycol), poly(ethylene glycol)-co-anhydride, poly(ethylene glycol)-co-lactide, poly(ethylene glycol)-co-glycolide, poly(ethylene glycol)-co-orthoester, poly-isopropylacrylamide, polyHEMA, polyacrylamide, calcium alginate, or a combination thereof;
- and wherein said detection compound is selected from the group consisting of the competitive binding pair FITC-Dextran and TRITC-ConA, or FITC-Dextran and TRITC-succinyl-ConA,
- (b) measuring the analyte levels.
Dependencies are inferred from the claim text.