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AssigneeTransgenomic, Inc.
InventorsDouglas T. Gjerde, Robert M. Haefele, Laura Bunnel, Paul D. Taylor, Kimberly A. Lamb, Satyajit Kar
Estimating the term…
An apparatus for effecting base pair length separations of DNA fragments by matched ion paired chromatography comprising a separation column containing separation media having non-polar DNA separation surfaces, separation solution supply means, and a separation solution conduit communicating with the separation column and the separation solution supply means, and a cleaning solution valve means positioned in the separation solution conduit for injecting cleaning solution into the separation solution conduit. A process for cleaning the non-polar DNA separation surfaces in the apparatus comprising interrupting the flow of separation solvent with a block of cleaning solution injected into the flow of separation solution passing to the column, the cleaning solution containing agent which removes accumulated residues from the non-polar surface. The cleaning solution can have an alkaline pH and contain a chelating agent such as EDTA.
A process for cleaning the non-polar polynucleotide separation surfaces in an apparatus for effecting separations of polynucleotide fragments by reverse phase ion pairing chromatography, the apparatus comprising a separation column containing separation media having non-polar polynucleotide separation surfaces, separation solution supply means, a separation solution conduit communicating with the separation column and the separation solution supply means, and a cleaning solution valve means positioned in the separation solution conduit for injecting cleaning solution into the separation solution conduit, the process comprising interrupting the flow of separation solvent with a block of cleaning solution injected into the flow of separation solution passing to the column, the cleaning solution containing agent which removes accumulated residues from the non-polar surface.
Dependencies are inferred from the claim text.