Antisense intron inhibition of starch branching enzyme expression
AssigneeDanisco A/S
InventorPeter Poulsen
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Abstract
A method for affecting enzymatic activity in a starch producing organism is described. The method comprises expressing in the organism: (a) a first nucleotide which comprises, partially or completely, a first intron of a gene encoding a class A starch branching enzyme in an antisense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron, (b) together with a second nucleotide sequence which comprises, partially or completely, a second intron of a class B starch branching enzyme in an antisense or sense orientation. Also described are antisense sequences, constructs, vectors, transformed cells, and transgenic organisms.
Claims10
A method for affecting starch branching enzyme activity in a starch producing plant cell, plant tissue, plant organ, or plant, comprising expressing in said plant cell, plant tissue, plant organ, or plant:
- (a) a first nucleotide sequence which comprises a first intron of a potato gene encoding a class A starch branching enzyme in an antisense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron,
- (b) linked to a second nucleotide sequence which comprises a second intron of a potato gene encoding a class B starch branching enzyme in an antisense or sense orientation.
An antisense sequence comprising a nucleotide sequence which is the complement of SEQ ID NO:38, or a nucleotide sequence with at least 95% similarity to SEQ ID NO:38.
A vector comprising a first nucleotide sequence which comprises a first intron of a potato gene encoding a class A starch branching enzyme in an antisense orientation, linked to a second nucleotide sequence which comprises a second intron of a potato gene encoding a class B starch branching enzyme in an antisense or a sense orientation, wherein the first nucleotide sequence does not contain a sequence that is antisense to an exon sequence naturally associated with the first intron.
Dependencies are inferred from the claim text.