Bacteriophage, a process for the isolation thereof, and a universal growth medium useful in the process thereof
AssigneeCouncil of Scientic and Industrial Research
InventorsPushpa Agrawal, Vishal Soni
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Abstract
The present invention provides a isolated bacteriophage useful as a tool for studying biological, biochemical, physiological and genetic properties of actinomycetes and other organisms which comprises a novel strain of Saccharomonospora having certain specified characteristics. The invention also relates to a process for the isolation of the said bacteriophage and/or DNA phage and to a novel universal growth medium which is particularly useful in the said process. Another embodiment of the process relates to a cloning vector which comprises a plasmid or bacteriophage comprising the phage DNA of the invention.
Claims33
A process for the isolation of a bacteriophage which comprises incubating a novel strain of Saccharomonospora deposited at DSMZ-DEUTSCHE SAMMELUNG VON MIKROORGANISMEN UND ZELLKULRTUREN GmbH bearing the accession number DSM 12317 (Indian Accession No. MTCC A0001) in a nutrient medium until the autolysis stage is reached, isolating and purifying from the medium a bacteriophage generated in the lysed culture.
An isolated bacteriophage having a double-stranded DNA genome of about 90 kilobase pairs with a (G+C) content of 69-71 mol percent, which infects the same range of species of bacteria as phase PIS 136.
An isolated bacteriophage isolatable from Saccharaomonospora, having a double-stranded DNA genome of about 90 kilobase pairs with a (G+C) content of 69-71 mol percent, which lysogenizes and integrates into the genome of actinomycetes.
An isolated bacteriophage isolatable from Sacharaomonospora, having a double-stranded DNA genome of about 90 kilobase pairs with a (G+C) content of 69-71 mol percent, which acts as a transposon in actinomycetes.
Dependencies are inferred from the claim text.