Nucleic acids that control seed and fruit development in plants
AssigneeThe Regents of the University of California
InventorsRobert L. Fischer, Yeonhee Choi, Mike Hannon
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Abstract
The invention provides methods of controlling endosperm and seed development in plants.
Claims38
An isolated nucleic acid compirsing a polynucleotide sequence, or complement thereof, encoding an ATR polypeptide exhibiting at least 80% sequence identity to SEQ ID NO:2, wherein the nucleic acid, when introduced into a plant to inhibit expression, results in a plant with increased endosperm development and wherein the ATR polypeptide comprises a leucine zipper and a nuclear localization signal sequence.
An expression cassette comprising a promoter operably linked to a heterologous polynucleotide sequence, or a complement thereof, encoding an ATR polypeptide exhibiting at least 80% sequence identity to SEQ ID NO:2, wherein the nucleic acid, when introduced into a plant to inhibit expression, results in a plant with increased endosperm development and wherein the ATR polypeptide comprises a leucine zipper and a nuclear localization signal sequence.
A host cell comprising an exogenous nucleic acid comprising a polynucleotide sequence, or complement thereof, encoding an ATR polypeptide exhibiting at least 80% sequence identity to SEQ ID NO:2, wherein the nucleic acid, when introduced into a plant to inhibit expression, results in a plant with increased endosperm development and wherein the ATR polypeptide comprises a leucine zipper and a nuclear localization signal sequence.
A method of modulating transiption of an ATR polynucleotide, the method comprising,
- introducing into a host cell an expression cassette comprising a promoter operably linked to an ATR polynucleotide, or complement thereof, encoding an ATR polypeptide at least 80% identical to SEQ ID NO:2, wherein the ATR polypeptide comprises a leucine zipper and a nuclear localization signal sequence;
- and
- detecting a host cell with modulated transciption of a polynucleotide encoding an ATR polypeptide at least 80% identical to SEQ ID NO:2 compared to a host cell wherein the expression cassette is not introduced.
A transgenic plant cell or transgenic plant comprising a heterologous nucleic acid comprising a polynucleotide sequence, or complement thereof, encoding an ATR polypeptide exhibiting at least 80% sequence identity to SEQ ID NO:2, wherein the nucleic acid, when introduced into a plant to inhibit expression, results in a plant with increased endosperm development and wherein the ATR polypeptide comprises a leucine zipper and a nuclear localization signal sequence.
A plant which has been regenerated from a plant cell according to 31.
Dependencies are inferred from the claim text.