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US 6,476,295 B2Granted
Expression of sucrose phosphorylase in plants
AssigneeMonsanto Technology LLC
InventorsGerard Francis Barry, Jan Willem de Weerd, Ganesh Murthy Kishore, Marcia Lee Weldon
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Abstract
Introducing sucrose phosphorylase activity into plants by transformation with a gene for the enzyme increases the rate of sucrose hydrolysis, leading to increased starch, oil, and protein levels. The preferred gene is from Streptococcus mutans. Surprisingly, in potatoes transformed to express this gene in tubers, reduced bruise discoloration susceptibility and increased uniformity of starch deposition throughout the tuber are achieved.
Claims5
A transformed plant cell comprising a recombinant, double-stranded DNA molecule comprising in sequence:
- (a) a promoter which functions in said plant cell;
- (b) a structural DNA sequence that causes the production of an RNA sequence which encodes a sucrose phosphorylase enzyme;
- and
- (c) a 3′ non-translated region which functions in plant cells to cause transcriptional termination and the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence.
1 more element
Dependencies are inferred from the claim text.