Plant phosphatidic acid phosphatases
AssigneeCalgene, Inc.
InventorsMichael W. Lassner, Diane M. Ruezinsky
Life of this patent
Estimating the term…
Abstract
By this invention, novel nucleic acid sequences encoding for phosphatidic acid phosphatase (PAP) proteins are provided, wherein said PAP protein is active in the formation of diacylglycerol from phosphatidic acid. Also considered are amino acid and nucleic acid sequences obtainable from PAP nucleic acid sequences and the use of such sequences to provide transgenic host cells capable of producing altered lipid compositions and total lipid levels.
Claims24
An isolated DNA sequence encoding a plant phosphatidic acid phosphatase protein, wherein said plant phosphatidic acid phosphatase protein is from Arabidopsis thaliana.
An isolated DNA sequence encoding a plant phosphatidic acid phosphatase protein, wherein said plant phosphatidic acid phosphatase protein is from corn.
An isolated DNA sequence encoding a plant phosphatidic acid phosphatase protein, wherein said plant phosphatidic acid phosphatase protein is from soybean.
A recombinant DNA construct comprising, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in a plant cell, a DNA structural gene sequence encoding an Arabidopsis thaliana phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
A plant cell having a DNA construct comprising, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in a plant cell, a DNA structural gene sequence encoding an Arabidopsis thaliana phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
A plant having a plant cell comprising a DNA construct that comprises, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in a plant cell, a DNA structural gene sequence encoding an Arabidopsis thaliana phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
A method of modifying the lipid composition in a plant cell, said method comprising:
- transforming a plant cell with DNA comprising as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region fictional in a plant cell, a DNA structural gene sequence encoding an Arabidopsis thaliana phosphatidic acid phosphatase, and a transcription termination sequence, capable of terminating transcription in said plant cell, and
- growing said plant cell under conditions wherein transcription of said Arabidopsis thaliana phosphatidic acid phosphatase is initiated,
- whereby said lipid composition is modified.
A recombinant DNA construct comprising, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in a plant cell, a DNA structural gene sequence encoding a corn phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
A recombinant DNA construct comprising, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in a plant cell, a DNA structural gene sequence encoding a soybean phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
A plant cell having a DNA construct comprising, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in said plant cell, a DNA structural gene sequence encoding a corn phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
A plant cell having a DNA construct comprising, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in said plant cell, a DNA structural gene sequence encoding a soybean phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
A plant having a plant cell comprising a DNA construct that comprises, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in said plant cell, a DNA structural gene sequence encoding a corn phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
A plant having a plant cell comprising a DNA construct that comprises, as operably linked in the 5′ to 3′ direction of transcription, a transcriptional initiation region functional in said plant cell, a DNA structural gene sequence encoding a soybean phosphatidic acid phosphatase, and a transcription termination sequence capable of terminating transcription in said plant cell.
Dependencies are inferred from the claim text.