True breeding transgenics from plants heterozygous for transgene insertions
AssigneeNew Zealand Institute for Food and Crop Research Limited
InventorAnthony John Conner
Life of this patent
Estimating the term…
Abstract
The invention provides a method for biasing a crop plant which is heterozygous for a transgene towards the production of seeds which carry the transgene comprising the step of contacting the crop plant containing a gene construct comprising the transgene coding for resistance to a specific phytotoxin with the specific phytotoxin one or more times during the life of the crop plant. The method achieves this through selective inhibition of phytotoxin-sensitive plant ovules, embryos and pollen. The method has particular application to the production of open pollinated and synthetic varieties of crop plants, such as alfalfa synthetic varieties.
Claims24
A method for biasing a crop plant which is heterozygous for a transgene coding for a protein that confers resistance to a translocatable herbicide towards the production of seeds which carry the transgene, comprising contacting the crop plant containing a gene construct comprising the transgene with the translocatable herbicide at two or more stages of commercial seed production, wherein the crop plant is part of an open-pollinated population or a synthetic variety and wherein a higher proportion of seed of the crop plant comprise the transgene as compared to seed of a plant that is not contacted with the herbicide.
A method for increasing the presence of a transgene that codes for a protein that confers resistance to a translocatable herbicide in commercial seed of a synthetic or an open pollinated population comprising:
- a) introducing one or more plants heterozygous for a transgene coding for a protein that confers resistance to a translocatable herbicide into a synthetic or open pollinated population;
- b) contacting the synthetic or open pollinated population produced in step a) with the translocatable herbicide at two or more stages of commercial seed production;
- and
- c) bulking the seed produced in step b) to produce commercial seed wherein a higher proportion of seed comprise the transgene as compared to seed of the synthetic or open pollinated population that is not contacted with the herbicide.
A method for biasing a crop plant which is heterozygous for a transgene coding for a protein that confers resistance to a translocatable herbicide towards the production of seeds which carry the transgene comprising contacting the crop plant containing a gene construct comprising the transgene with the translocatable herbicide more than once during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety and wherein a higher proportion of seed of the crop plant comprise the transgene as compared to seed of a plant that is not contacted with the herbicide.
A method of selectively inhibiting herbicide-sensitive plant ovules, embryos and/or pollen in order to bias a crop plant which is heterozygous for a transgene coding for a protein that confers resistance to a translocatable herbicide towards the production of seeds which carry the transgene comprising contacting the crop plant containing a gene construct comprising the transgene with the translocatable herbicide more than once during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety and wherein said contacting results in selective inhibition of herbicide sensitive plant ovules, embryos, and/or pollen, and a consequent higher proportion of seed comprise the transgene as compared to seed of the synthetic or open pollinated population that is not contacted with the herbicide.
A method of selectively inhibiting herbicide-sensitive plant ovules in order to bias a crop plant which is heterozygous for a transgene coding for a protein that confers resistance to a translocatable herbicide towards the production of seeds which carry the transgene comprising contacting the crop plant containing a gene construct comprising the transgene with the translocatable herbicide more than once during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety and wherein said contacting results in selective inhibition of herbicide-sensitive plant ovules and a consequent higher proportion of seed of the crop plant comprise the transgene as compared to seed of a plant that is not contacted with the herbicide.
A method of selectively aborting herbicide-sensitive plant embryos in order to bias a crop plant which is heterozygous for a transgene coding for a protein that confers resistance to a translocatable herbicide towards the production of seeds which carry the transgene comprising contacting the crop plant containing a gene construct comprising the transgene with the translocatable herbicide more than once during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety and wherein said contacting results in selective abortion of herbicide-sensitive plant embryos and a consequent higher proportion of seed of the crop plant comprise the transgene as compared to seed of a plant that is not contacted with the herbicide.
A method of selectively inhibiting herbicide-sensitive pollen in order to bias a crop plant which is heterozygous for a transgene coding for a protein that confers resistance to a translocatable herbicide towards the production of seeds which carry the transgene comprising contacting the crop plant containing a gene construct comprising the transgene with the translocatable herbicide more than once during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety and wherein said contacting results in selective inhibition of herbicide-sensitive pollen and a consequent higher proportion of seed of the crop plant comprise the transgene as compared to seed of a implant that is not contacted with the herbicide.
A method of producing a new synthetic alfalfa variety comprising the step of:
- 1) producing a transgenic alfalfa plant, wherein the transgenic alfalfa plant is heterozygous for an introduced gene construct which comprises a heterologous gene coding for a protein that confers translocatable herbicide resistance operably linked to a heterologous gene of interest;
- 2) using the transgenic alfalfa plant produced by step 1) as a parent and crossing the transgenic alfalfa plant to one or more alfalfa plants which do not contain the translocatable herbicide resistance gene operably linked to the heterologous gene of interest;
- 3) applying the translocatable herbicide more than once during the life cycle of the plants in step 2);
- and
- 4) harvesting and bulking the resultant seed to produce a new synthetic alfalfa variety wherein a higher proportion of seed of the new synthetic alfalfa variety contain the translocatable herbicide resistance gene operably linked to the gene of interest as compared to seed of plants that are not contacted with the herbicide.
Dependencies are inferred from the claim text.