Safe pharmaceutical composition for treating and preventing infertility and increasing immune function

A process for producing Icariin (ICN), which used for treating infertility and increasing immune function comprises:

• a. extracting said Epimedium brevicornum Maxim., Epimedium sagittatum Maxim;
• Epimedium pubescens Maxim., Epimedium wushanense T. S. Ying, and Epimedium Koreanum Nakai with 95% of ethanol;
• b. the ethanol extraction was recovered and the residue obtained;
• c. the residue extracted by 95% of petroleum ether, CH2Cl2, acetic ether and n-butyl alcohol sequentially;
• d. n-butyl alcohol extraction was recovered and residue obtained;
• e. the residue was passed through a column of polyamide and elute with ethanol;
• f. 10% of ethanol elute was passed through a column of silica gel and elute with CHCl3—CH3OH3;
• g. the CCl3—CH3OH solution concentrated under reduced pressure and the crystal was obtained;
• and
• h. the crystal was recrystalized from CH3OH, and
• i. the final product was Icariin.