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AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsKozo Yamamoto, Iwao Okamoto, Masashi Kurimoto
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Disclosed are created stable polypeptides which are capable of inducing the production of interferon-gamma by immunocompetent cells. The present polypeptides contain specific amino acid sequences usually derived from the wild-type polypeptides, being capable of the production of interferon-gamma, by replacing the cysteine(s) with different amino acid(s). The present polypeptides possess a stability and an activity of inducing the production of IFN-γ by immunocompetent cells, both of which are significantly higher than those of the wild-type polypeptides. In addition to the activity, the present polypeptides can exhibit remarkable activities of inducing the formation of killer cells and enhancing thier cytotoxicities. The present polypeptides are easily obtainable by the process according to the present invention using recombinant DNA techniques. Thus the present polypeptides are useful for agents to treat and/or prevent susceptive diseases such as viral diseases, infections, malignant tumors, and immunopathies.
An artificial polypeptide derived from a wild-type polypeptide capable of inducing the production of interferon-γ by immunocompetent cells and comprising the amino acid sequence of either SEQ ID NO:4 or SEQ ID NO:5, said artificial polypeptide having the activity of inducing the production of interferon-γ by immunocompetent cells and consisting of a 157-amino acid sequence which comprises (i) the amino acid sequences of SEQ ID NOS:
An artificial polypeptide produced from the wild-type polypeptide consisting of the amino acid sequence of SEQ ID NO:4 by replacing one or more cysteine residues in the wild-type polypeptide sequence with an amino acid residue(s) selected from the group consisting of serine, threonine, alanine, valine, leucine, isoleucine, histidine, tyrosine, phenylalanine, tryptophan, and methionine, said artificial polypeptide having the activity of inducing the production of interferon-γ by immunocompetent cells.
An artificial polypeptide produced from, wherein the wild-type polypeptide consisting of the amino acid sequence of SEQ ID NO:5 by replacing one or more cysteine residues in the wild-type polypeptide sequence with an amino acid residue(s) selected from the group consisting of serine, threonine, alanine, valine, leucine, isoleucine, histidine, tyrosine, phenylalanine, tryptophan, and methionine, said artificial polypeptide having the activity of inducing the production of interferon-γ by immunocompetent cells.
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