Method for folding unfolded proteins
AssigneeNational Research Council of Canada
InventorsDaniel C. Tessier, Andrë Zapun, John J. M. Bergeron, David Y. Thomas
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Abstract
The present invention relates to methods of treating and diagnosing protein trafficking disorders and controlling secretory protein production. The present invention also relates to methods for folding unfolded proteins, especially in heterelogous expression systems. The methods comprise exposing an unfolded protein to a biological preparation comprising ERp57 in combination with calnexin or calreticulin under conditions to permit folding of the unfolded protein.
Claims4
A method for folding unfolded proteins or for enhancing the formation of folded proteins which comprises a step of exposing an unfolded protein to a biological preparation comprising an ERp57 specifically bound to calnexin or calreticulin under conditions to permit folding of the unfolded protein.
A method for forming disulfide bonds in an unfolded protein or for enhancing the formation of disulfide bonds in an unfolded protein which comprises a step of exposing an unfolded protein to a biological preparation comprising an ERp57 specifically bound to calnexin or calreticulin under conditions to permit folding of the unfolded protein.
Dependencies are inferred from the claim text.