Recombinant antigens useful for the serodiagnosis of neosporosis
OwnerNicola C. Lally, Mark C. Jenkins +1inventors
InventorsNicola C. Lally, Mark C. Jenkins, Jitender P. Dubey
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Abstract
Antigens for the detection of antibodies to Neospora parasites for the diagnosis of neosporosis have been identified. Recombinant antigens may be produced by expression of DNA sequences derived from Neospora caninum. Both antigens are capable of detecting antibody responses in animals experimentally inoculated with N. caninum but show no evidence of cross-reactivity with serum from animals inoculated with closely related parasites such as Toxoplasma gondii or Sarcocystis species.
Claims8
An isolated and purified antigenic protein which specifically detects antibodies to Neospora, said protein comprising an approximately 35-kDa protein which is immunologically reactive with antibodies to Neospora and comprises the amino acid sequence of SEQ ID NO:
- 2 or a sequence having substantial homology thereto, or a portion of said protein which is immunologically reactive with antibodies to Neospora and comprises a fragment of SEQ ID NO:
- 2 or a sequence having substantial homology thereto, said fragment having at least one epitope which binds to said antibodies.
An isolated and purified antigenic protein which specifically detects antibodies to Neospora, said protein comprising an approximately 30-kDa protein which is immunologically reactive with antibodies to Neospora and comprises the amino acid sequence of SEQ ID NO:
- 4 or a sequence having substantial homology thereto, or a portion of said protein which is immunologically reactive with antibodies to Neospora and comprises a fragment of SEQ ID NO:
- 4 or a sequence having substantial homology thereto, said fragment having at least one epitope which binds to said antibodies.
Dependencies are inferred from the claim text.