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US 6,476,190 B1Granted
Process for preparing modified proteins
AssigneeThe Scripps Research Institute
InventorsStephen B. H. Kent, Martina Schnolzer-Rackwitz
Life of this patent
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Abstract
Processes for the preparation of modified proteins comprising the coupling of a first peptide segment having a haloacyl group at the N-terminus thereof with a second peptide sequent having a carbonylthiol group at the C-terminus thereof are disclosed. Novel modified proteins produced by the process are also disclosed.
Claims5
A process for preparing a peptide-containing or pseudopeptide-containing biomolecule which comprises the steps of:
- a. providing a first segment selected from the group consisting of peptides and pseudopeptides, said first segment containing at least one non-coded structural unit and having a terminal position with a first chemoselective synthon having a carbonylthiol or carbonylseleno functionality;
- b. providing a second segment selected from the group consisting of peptides and pseudopeptides, said second segment optionally containing at least one non-coded structural unit and having a terminal position with a second chemoselective synthon, the second chemoselective synthon having an α-halo carbonyl functionality complementary to the first chemoselective synthon of the first segment;
- and
- c. ligating the first segment and the second segment, whereby the first chemoselective synthon at the terminal position of the first segment and the second chemoselective synthon at the terminal position of the second segment form a pseudopeptide backbone linkage;
- wherein the pseudopeptide backbone linkage is selected from the group consisting of:
- —CO—S—CH2— and —CO—Se—CH2—;
- and
- wherein the first segment and second segments each include from two to one hundred amino acid residues;
- and then
- d. isolating the ligation product of said Step C for obtaining the peptide-containing or pseudopeptide-containing biomolecule.
7 more elements
A process for preparing a peptide-containing or pseudopeptide-containing biomolecule which comprises the steps of:
- a. providing a first segment selected from the group consisting of peptides and pseudopeptides, said first segment containing at least one non-coded structural unit and having a terminal position with a first chemoselective synthon having a carbonylthiol functionality;
- b. providing a second segment selected from the group consisting of peptides and pseudopeptides, said second segment optionally containing at least one non-coded structural unit and having a terminal position with a second chemoselective synthon, the second chemoselective synthon having an α-halo carbonyl functionality complementary to the first chemoselective synthon of the first segment;
- and
- c. ligating the first segment and the second segment, whereby the first chemoselective synthon at the terminal position of the first segment and the second chemoselective synthon at the terminal position of the second segment form a pseudopeptide backbone linkage;
- wherein the pseudopeptide backbone linkage is represented by the following formula:
- —CO—S—CH2—;
- and
- wherein the first segment and second segments each include from two to one hundred amino acid residues;
- and then
- d. isolating the ligation product of said Step C for obtaining the peptide-containing or pseudopeptide-containing biomolecule.
7 more elements
A process for preparing a peptide-containing or pseudopeptide-containing biomolecule which comprises the steps of:
- a. providing a first segment selected from the group consisting of peptides and pseudopeptides, said first segment containing at least one non-coded structural unit and having a terminal position with a first chemoselective synthon having a carbonylseleno functionality;
- b. providing a second segment selected from the group consisting of peptides and pseudopeptides, said second segment optionally containing at least one non-coded structural unit and having a terminal position with a second chemoselective synthon, the second chemoselective synthon having an α-halo carbonyl functionality complementary to the first chemoselective synthon of the first segment;
- and
- c. ligating the first segment and the second segment, whereby the first chemoselective synthon at the terminal position of the first segment and the second chemoselective synthon at the terminal position of the second segment form a pseudopeptide backbone linkage;
- wherein the pseudopeptide backbone linkage is represented by the following formula:
- —CO—Se—CH2—;
- and
- wherein the first segment and second segments each include from two to one hundred amino acid residues;
- and then
- d. isolating the ligation product of said Step C for obtaining the peptide-containing or pseudopeptide-containing biomolecule.
7 more elements
Dependencies are inferred from the claim text.