Protein arrays for high-throughput screening

A method for screening a plurality of proteins for interactions with one or more components of a fluid sample comprising the steps of:

• providing a substrate having a surface and an array of spaced-apart protein immobilization regions over said surface, said protein immobilization regions being effective to immobilize one or more selected proteins, and one or more border regions surrounding each protein immobilization region and separating such protein immobilization regions from one another, said border regions each comprising (i) an ordered hydrophobic monolayer formed of alkyl chains having proximal ends being chemisorbed or physisorbed to said surface within said protein immobilization regions, and opposite hydrophobic distal ends, (ii) a hydrophilic monolayer attached to said hydrophobic monolayer, said hydrophilic monolayer comprising hydrophilic chains, each hydrophilic chain having a proximal end by which said hydrophilic chain is linked to an alkyl chain distal end, and an opposite hydrophilic distal end, together said hydrophobic monolayer and hydrophilic monolayers forming said border regions effectively resistant to non-specific protein binding;
• applying said selected proteins to said protein immobilization regions to form an array of protein regions having one or more selected proteins displayed within said protein immobilization regions such that each protein immobilization region is separated from other protein immobilization regions by border regions resistant to non-specific protein binding;
• exposing said protein regions to said fluid sample;
• and,
• detecting said interaction(s), if any within said protein regions.