P element derived vector and methods for its use
AssigneesThe Board of Trustees of the Leland Stanford Junior University, Tosk, Inc.
InventorsPatrick Fogarty, Joseph Lipsick
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Abstract
Novel P element derived vectors and methods for their use to insert an exogenous nucleic acid into the genome of a target cell are provided. The subject vectors have a pair of P element transposase recognized insertion sites, e.g. 31 base pair inverted repeats, flanking at least two transcriptionally active genes. In practicing the subject methods, a vector of the subject invention is introduced into the target cell under conditions sufficient for transposition to occur. The subject methods find use in a variety of applications in which the insertion of an exogenous nucleic acid into the genome of a target cell is desired, e.g. include research, synthesis and therapeutic applications.
Claims17
A P element vector for introducing an exogenous nucleic acid into a target non-insect cell genome, said vector comprising:
- a pair of P element transposase recognized insertion sequences flanking at least two transcriptionally active genes.
A method of inserting an exogenous nucleic acid into a genome of a non-insect target cell, said method comprising:
- introducing into said non-insect target cell a P-element vector comprising said exogenous nucleic acid under conditions sufficient for transposition to occurs so that said exogenous nucleic acid is inserted into said genome of said non-insect target cell.
Dependencies are inferred from the claim text.