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US 6,475,790 B2Granted
Assay for screening agents that alter angiogenesis
AssigneeBioCure, Inc.
InventorsEileen T. Grant, Graham T. Bell, Stephen Bloor
Life of this patent
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Abstract
A multicellular in vitro assay which models the combined stages of angiogenesis is disclosed. The assay permits the evaluation of drug candidates for their potential utility in treating pathological conditions such as chronic dermal ulcers, tumors, diabetic retinopathy, psoriasis and inflammation.
Claims7
A multicellular in vitro assay method for screening agents that alter angiogenesis comprising:
- (a) mixing a suspension of human fibroblasts with a suspension of human umbilical vein endothelial cells in a ratio of from about 2:1 to 8:1 to provide a mixture of cells, both of said endothelial cells and said fibroblasts being suspended in endothelial growth medium;
- (b) dividing said mixture of cells into at least two test containers;
- (c) adding an agent to be screened to at least one of said containers;
- (d) culturing said mixture of cells, whereby the fibroblasts form a monolayer in which the endothelial cells display proliferation, migration and differentiation into networks of tubules in at least one of said containers to which said agent has not been added;
- and
- (e) comparing said at least two test containers.
3 more elements
Dependencies are inferred from the claim text.