Human platelet-derived growth factor receptor
AssigneeThe Regents of the University of California
InventorsJaime A. Escobedo, Lewis T. Williams
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Abstract
A DNA sequence encoding human platelet-derived growth factor receptor (hPDGF-R) has now been isolated and sequenced. An expression construct comprising the sequence encodes a receptor that can be secreted or incorporated into the membrane of a mammalian cell. The incorporated receptor is functionally equivalent to the wild-type receptor, conferring a PDGF-sensitive mitogenic response on cells lacking the receptor. The construct can be used for enhancing PDGF response of cells, determining the regions involved in transducing the signal in response to PDGF binding, providing mutated analogs and evaluating drugs for their physiologic activity.
Claims18
A hPDGF-R fragment having PDGF receptor binding activity, said fragment consisting essentially of amino acids beginning at about 33 through about 500 of the external domain of hPDGF-R.
A hPDGF-R fragment having tyrosine kinase activity said fragment consisting essentially of the intracellular portion of a hPDGF-R.
A cell producing a substantially pure type A hPDGF-R polypeptide comprising an intracellular or extracellular domain of the amino acid sequence shown in FIG. 2 wherein the polypeptide is less than full-length.
Dependencies are inferred from the claim text.