US 6,475,774 B1 · Reaction plate sealing means

Abstract

The invention comprises a wide range of pierceable and memory-recloseable septa cover for multi-well plates for PCR or other small sample well processes, where such seals are joined into an easily applied or removed sheet or otherwise joined means. A reaction chamber well comprises a liquid level portion at the bottom that contains the various levels of reaction liquid, a super-liquid portion comprising the gas volumes above the reaction liquid. In one form of the invention embodiments, the cover comprises a gas tight seal between the cover and the side of the well substantially above the liquid level, thereby forming the super-liquid space. The disturbance, pressure change and/or gas exchange in this small gas space results in a rather dramatic potential change in reaction or mixing results. The invention cover reduces each of these to a minimum not attainable in the prior art.

Claims18

Dependent claims

A sealing device adapted to seal one or more wells on a PCR plate comprising:

(a) a support surface adapted to sealingly support around a peripheral edge a concave and downward extension of the support surface;
(b) the extension comprising a bottom portion having a bottom thickness and an impression along a lowest part of the bottom portion, the impression comprising an impression thickness at the bottom of the impression, such that the impression thickness is substantially less than the bottom thickness;
(c) an outer surface of an upper part of the extension is adapted to sealingly engage a top and inner rim part of a PCR plate well;
and
(d) at least the bottom portion comprises a rubber or elastomer material adapted to be pierced by a pipette through the area of impression thickness.

2 more elements

Dependencies are inferred from the claim text.

Reaction plate sealing means

Life of this patent

Reaction plate sealing means

Life of this patent

Abstract

Claims18