Strain of Trichoderma harzianum useful as nematode inhibitor, fungicide and plant growth promoter and a process for the isolation thereof

A process for the isolation of a biologically pure stain of Trichoderma harzianuzn having fungicidal activity against phytopathogenic fungus, having inhibitor activity against phytonematode, and having plant growth promoter activity, and which has ATCC accession No. PTA-3701, said process comprising:

• (a) obtaining a spore suspension having 103 to 108 cells/ml. of Trichoderma harzianrum isolated from experimental fields of Central Institute of Medicinal and Aromai Plants (CIMAP) Field Station, Bangalore, India;
• (b) treating the spore suspension with 100-500 ppm of ethyl methyl sulphonate rnutagen for 0.5 to 6 hours;
• (c) washing the treated spores with sterilized distilled water and placing the spores in Petri plates containing a growth medium for the spores;
• and (d) selecting and isolating a whitish and fast growing strain of Trichoderma harzianum as said biologically pure strain of Trichoderma harzianum.