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AssigneeForskarpatent I Syd AB
InventorsRicardo Román Cordero Otero, Márk Gárdonyi, Bärbel Hahn-Hägerdal, Willem Heber van Zyl, Eva Anna Viktoria Dackehag
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The present invention relates to novel nucleic acid sequences which, upon expression in a procaryotic or eucaryotic cell, code for a polypeptide having an elevated specific xylose isomerase activity compared to the wildtype Thermus thermophilus xylose isomerase. They are selected from a) nucleic acid sequences shown in SEQ. ID. No 1; b) the complementary strand of the sequence defined in (a) above; c) nucleic acid sequences which hybridize to the sequences defined in (a) or (b) above; d) nucleic acid sequences which, but for the degeneracy of the genetic code, would hybridize to the sequences defined in (a), (b) or (c) above and which code for the same polypeptide as those defined in (a), (b) or (c) above. The present invention further provides a process of producing ethanol from xylose containing materials comprising contacting cells that express such nucleic acid sequences and novel modified xylose isomerases that advantageously can be applied in the production of fructose syrups.
An isolated nucleic acid encoding a polypeptide comprising an amino acid sequence encoded by SEQ. ID. No. 1,
An isolated nucleic acid having a nucleotide sequence that is at least 85%, but less than 100%, identical to the nucleotide sequence of SEQ. ID. No. 2,
Dependencies are inferred from the claim text.