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US 6,475,767 B1Granted
Process for producing L-methionine γ-lyase crystals
AssigneeShionogi & Co., Ltd.
InventorsKensaku Akita, Tomoaki Takakura, Akio Takimoto, Takaomi Ito
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Abstract
A process for production of L-methionine γ-lyase crystals by using polyethylene glycol, characterized by comprising the first step of warming a solution containing L-methionine γ-lyase before or after addition of polyethylene glycol thereto and the second step of adding an inorganic salt.
Claims20
process for producing L-methionine γ-lyase crystals which comprises
- dissolving precipitate containing L-methionine γ-lyase in a buffered solution at a temperature in the range of about 2° C. to about 15° C. to form a solution containing L-methionine γ-lyase,
- adding polyethylene glycol to the solution and warming the solution to a temperature in the range of about 25° C. to about 40° C., and
- adding an inorganic salt to the warm solution to promote formation of L-methionine γ-lyase crystals.
Dependencies are inferred from the claim text.