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AssigneeMicronas GmbH
InventorsWerner Baumann, Ralf Ehret, Mirko Lehmann, Günter Igel, Hans-Jürgen Gahle, Bernhard Wolf, Ulrich Sieben, Ingo Freund, Martin Brischwein
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A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.
A process for intracellular manipulation of at least one biological cell (3) present in a culture medium and adherently deposited on a support area, comprising creating an opening in a membrane of the cell (3) and manipulating the cell interior through this opening, wherein the open is created in the cell membrane within the support area (5) for the cell (3) and at a distance from an edge of the support, and wherein the opening in the membrane of the cell is created by means selected from the group consisting of electroporation, mechanical impulse, irradiation, chemical poration and electrical, chemical or radiation activation of an activatable poration substance.
Dependencies are inferred from the claim text.